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F(ab')2 Human IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

F(ab')2 Human IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Catalog Number: orb348215

DispatchUsually dispatched within 5-10 working days
$ 580.00
Catalog Numberorb348215
CategoryAntibodies
DescriptionF(ab')2 Human IgG (H&L) antibody (FITC)
ClonalityPolyclonal
Species/HostDonkey
IsotypeIgG F(ab')2
ConjugationFITC
ReactivityHuman
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
ImmunogenHuman IgG whole molecule
Tested applicationsFC, FLISA, IF
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Application notesF(ab')2 Anti-Human IgG (H&L) Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesDonkey F(ab')2 Anti-Human IgG fluorescein Conjugat
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NoteFor research use only