F(ab')2 Human IgG F(ab')2 Antibody Pre-Adsorbed

SKU: orb348178

Description

F(ab')2 Human IgG F(ab')2 antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:25,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  F(ab')2 Anti-Human IgG F(ab')2 Antibody has been tested by ELISA, SDS-PAGE, and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Anti-Human IgG was produced by repeated immunization with human IgG F(ab')2 fragment in goat.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody Pre-Adsorbed, Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody

SDS-PAGE results of Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody. Lane 1: reduced Goat F(ab')2 Anti-Human IgG F(ab')2. Lane 2: Opal PreStained Molecular Weight Ladder. Lane 3: non-reduced Goat F(ab')2 Anti-Human IgG F(ab')2. Load: 1.0 µg. 4-20% SDS Gel, Coomassie Blue Stained.