F(ab')2 Human IgG Fc AntibodyPre-Adsorbed

• Catalog Number: orb348177
• Category: Antibodies
• Description: F(ab')2 Human IgG F(c) antibody
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG F(ab')2
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins.
• Immunogen: Human IgG F(c) fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:1,500 - 1:7,000, IHC: 1:1,000 - 1:5,000, WB: 1:500 - 1:5,000
• Application notes: F(ab')2 Anti-Human IgG F(c) Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: Goat F(ab')2 Anti-Human IgG F(c) Antibody Pre-Adso
• Note: For research use only

Western Blot of Goat anti-F(ab')2 Human IgG F(c) Antibody Pre-Adsorbed. Lane 1: Human IgG F(c) (p/n orb346220). Load: 100 ng per lane. Primary antibody: F(ab')2 Human IgG F(c) Antibody Pre-Adsorbed at 1:1000 o/n at 4°C. Secondary antibody: DyLight™ 800 Donkey anti-goat at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.