F(ab')2 Horse IgG (H&L) Antibody Biotin Conjugated

• Catalog Number: orb348157
• Category: Antibodies
• Description: F(ab')2 Horse IgG (H&L) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG F(ab')2
• Conjugation: Biotin
• Reactivity: Equine
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Horse IgG and Horse Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Immunogen: Horse IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:300,000, IHC: 1:1,000 - 1:2,500, WB: 1:10,000 - 1:25,000
• Application notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Rabbit F(ab')2 Anti-Horse IgG Antibody Biotin Conj
• Note: For research use only