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Catalog Number | orb348131 |
---|---|
Category | Antibodies |
Description | F(ab')2 Guinea Pig IgG (H&L) antibody (FITC) |
Clonality | Polyclonal |
Species/Host | Goat |
Isotype | IgG F(ab')2 |
Conjugation | FITC |
Reactivity | Guinea pig |
Form/Appearance | Lyophilized |
Concentration | 1.0 mg/mL |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
Immunogen | Guinea Pig IgG whole molecule |
Tested applications | FC, FLISA, IF |
Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
Application notes | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
Antibody Type | Secondary Antibody |
Biological Activity | 2.46 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Alternative names | Goat F(ab')2 Anti-Guinea Pig IgG Antibody Fluoresc Read more... |
Note | For research use only |
FC, FLISA, IF | |
Guinea pig | |
Rabbit | |
Polyclonal | |
FITC |
FC, FLISA, IF | |
Guinea pig | |
Rabbit | |
Polyclonal | |
FITC |