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Catalog Number | orb345924 |
---|---|
Category | Antibodies |
Description | F(Ab')2 antibody (Alkaline Phosphatase) |
Clonality | Polyclonal |
Species/Host | Goat |
Isotype | IgG F(ab')2 |
Conjugation | AP |
Form/Appearance | Liquid (sterile filtered) |
Concentration | 1.0 mg/mL |
Buffer/Preservatives | 0.1% (w/v) Sodium Azide |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
Immunogen | Fluorescein conjugated to Goat IgG |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:38,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
Application notes | F(ab')2 Anti-Fluorescein Antibody Conjugated to Alkaline Phosphatase has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
Antibody Type | Primary Antibody |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Alternative names | goat F(ab')2 anti-Fluorescein Antibody alkaline ph Read more... |
Note | For research use only |