F(ab')2 Dog IgG (H&L) Antibody Peroxidase Conjugated

• Catalog Number: orb346751
• Category: Antibodies
• Description: F(ab')2 Dog IgG (H&L) antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine
• Isotype: IgG F(ab')2
• Immunogen: Dog IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: F(ab')2 Anti-DOG IgG Antibody is Peroxidase Conjugated. The F(ab’)2 fragment of a rabbit IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG and Dog Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: Rabbit F(ab')2 anti-Dog IgG Antibody Peroxidase Co
• Note: For research use only
• Application notes: Dog antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for anti-dog antibody. F(ab')2 Anti-Dog IgG is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.