F(ab')2 Chicken IgG (H&L) Antibody Phycoerythrin conjugated

SKU: orb348096

Description

F(ab')2 Chicken IgG (H&L) antibody (RPE)

Images & Validation

• Tested Applications: FC, IF
• Dilution range: FC: 1:100 - 1:250, IF: 1:100 - 1:250
• Reactivity: Gallus
• Application Notes:
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Chicken IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Alternative Names

Rabbit F(ab')2 Anti-Chicken IgG Phycoerythrin Conjugated Antibody, Rabbit Fab2 Anti Chicken Phycoerythrin Conjugated Antibody, Rabbit Fab'2 Anti-Chicken Antibody PE Conjugation, Rabbit F(ab')2 Anti-Chicken IgY Phycoerythrin