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Fab Sheep IgG (H&L) Antibody Rhodamine Conjugated
Catalog Number: orb348506
| Catalog Number | orb348506 |
|---|---|
| Category | Antibodies |
| Description | Fab Sheep IgG (H&L) antibody (TRITC) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG Fab |
| Conjugation | TRITC |
| Reactivity | Sheep |
| Form/Appearance | Lyophilized |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c). |
| Immunogen | Sheep IgG whole molecule |
| Tested applications | FC, FLISA, IF, WB |
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Application notes | Fab Anti-Sheep IgG Rhodamine Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
| Antibody Type | Secondary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | Rabbit Fab Anti-Sheep IgG Antibody Rhodamine Conju Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Rhodamine conjugated Rabbit Fab Anti-Sheep IgG secondary antibody. Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Sheep IgG. Other band(s): None.
