Fab Mouse IgG (H&L) Antibody Peroxidase Conjugated

• Catalog Number: orb348473
• Category: Antibodies
• Description: Fab Mouse IgG (H&L) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG Fab
• Conjugation: HRP
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Immunogen: Anti-Mouse IgG (H&L) was produced by repeated immunization with mouse IgG whole molecule in goat.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:20,000
• Application notes: Fab Anti-Mouse IgG (H&L) peroxidase conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat Fab Anti-Mouse IgG Antibody Peroxidase Conjug
• Note: For research use only

Western blot of Peroxidase conjugated Goat Fab Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.