Fab Mouse IgG (H&L) Antibody Fluorescein Conjugated

• Catalog Number: orb348472
• Category: Antibodies
• Description: Fab Mouse IgG (H&L) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG Fab
• Conjugation: FITC
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Immunogen: Mouse IgG whole molecule
• Tested applications: DOT, IF, WB
• Dilution range: IF: 1:500 - 1:2,500
• Application notes: Fab Anti-Mouse IgG (H&L) Fluorescein Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat Fab Anti-Mouse IgG Antibody Fluorescein Conju
• Note: For research use only

Dot Blot results of Goat Fab Anti-Mouse IgG Antibody Fluorescein Conjugated. Dots are Mouse IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Goat Anti-Mouse IgG FITC at 1 µg/ml for 1hr at RT. Secondary Antibody: none.