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Fab Mouse IgG (H&L) Antibody Biotin Conjugated

Catalog Number: orb348474

DispatchUsually dispatched within 5-10 working days
$ 430.00
Catalog Numberorb348474
CategoryAntibodies
DescriptionFab Mouse IgG (H&L) antibody (Biotin)
ClonalityPolyclonal
Species/HostGoat
IsotypeIgG Fab
ConjugationBiotin
ReactivityMouse
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
ImmunogenMouse IgG whole molecule
Tested applicationsDOT, ELISA, IHC, WB
Dilution rangeELISA: 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Application notesFab Anti-Mouse IgG (H&L) Biotin Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesGoat Fab Anti-Mouse IgG Biotin Conjugated Antibody
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NoteFor research use only
Fab Mouse IgG (H&L) Antibody Biotin Conjugated

ELISA Results of Fab Anti-Mouse IgG (Goat) Antibody Biotin Conjugated. Each well was coated in duplicate with 10 µg of Mouse IgG (p/n orb2652749). The working dilution is 479000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS, Streptavidin-HRP conjugated (p/n orb348767) and TMB substrate (p/n orb348651).