F8/FVIII/Factor VIII Antibody

SKU: orb1537955

Description

F8/FVIII/Factor VIII Antibody. FVIII is a large molecule glycoprotein composed of 2,332 amino acids and is divided into multiple functional domains (arranged in the order of A1-A2-B-A3-C1-C2).

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P
• Dilution Range: ELISA, IHC, IHC-P (5 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

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Key Properties

• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human Factor VIIIC (F. VIII) purified from F. VIII concentrate.
• Target: F8 / FVIII / Factor VIII
• Purification: Caprylic acid and ammonium sulfate precipitations and solid-phase adsorption
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 10 mM HEPES, pH 7.4, 0.15 M NaCl, 50% Glycerol
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

F8, AHF, Antihemophilic factor, Coagulation factor VIIIc, Coagulation factor VIII, DXS1253E, Factor VIII F8B, FVIII, HEMA, F8B, Factor VIII, Procoagulant component, F8C

Quick Database Links

Gene Symbol

F8 / FVIII / Factor VIII