Ezrin (phospho Thr566) rabbit pAb

• Catalog Number: orb770375
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Ezrin (phospho-Thr566)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Thr566. AA range:534-583
• MW: 69
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-EZR antibody, anti-VIL2 antibody, anti-Ezrin
• Note: For research use only

Western blot analysis of HELA COLO using Phospho-Ezrin (T566) antibody. Antibody was diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ezrin (Phospho-Thr566) Antibody.

Western blot analysis of lysates from Jurkat cells treated PMA 125ng/ml 30', using Ezrin (Phospho-Thr566) Antibody. The lane on the right is blocked with the phospho peptide.