EZH2 Mouse Monoclonal Antibody

SKU: orb783423

Description

EZH2 Mouse Monoclonal Antibody

Research Area

Epigenetics, Polycomb Repressive Complex 2 (PRC2)

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,IgK
• Clone No.: E3H5
• Immunogen: Recombinant human EZH2
• Target: EZH2
• Molecular Weight: 82 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Enhancer of zeste homolog 2; Enx1h; MGC9169; Enhancer of zeste 2; ENX-1; ENX 1; ENX1; EZH 2; EZH2_HUMAN; Histone-lysine N-methyltransferase EZH2; KMT6A; Lysine N-methyltransferase 6; Enhancer of zeste homolog 2(Drosophila); Enhancer of zeste, Drosophila, homolog 2; KMT 6; KMT6A; KMT6; WVS2.

Blank control (black line): Jurkat. Primary Antibody (green line): Mouse Anti-EZH2 antibody (orb783423), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (EZH2) monoclonal Antibody, Unconjugated (orb783423) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes. Dylight® 554 Phalloidin was used to stain the cell Cytoplasmic.

Sample: Lane 1: Hela cell lysates, Lane 2: HL60 cell lysates, Lane 3: T47D cell lysates, Primary: Anti-EZH2 (orb783423) at 1:2000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 82 kD, Observed band size: 100 kD.

U251 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (EZH2) monoclonal Antibody, Unconjugated (orb783423) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes. Dylight® 554 Phalloidin was used to stain the cell Cytoplasmic.

Quick Database Links

Gene Symbol

EZH2

UniProt

Q15910