EXOG Antibody

• Catalog Number: orb1248042
• Category: Antibodies
• Description: EXOG Antibody
• Target: EXOG
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-KAKEQSGTQIRKP
• UniProt ID: Q9Y2C4
• MW: Approx 40kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell lines HepG2 and Jurkat (calculated MW of 41.1kDa according to NP_005098.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EXOG, endo/exonuclease (5'-3'), endonuclease G-lik
• Research Area: Cancer Biology, Metabolism Research, Neuroscience,
• Note: For research use only
• NCBI: NP_001138936.1, NP_005098.2

orb1248042 (0.2 ug/ml) staining of HepG2 (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248042 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.