EXO1 Antibody

SKU: orb1258378

Description

EXO1 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 601-846 of human EXO1 (NP_006018.4).
• Target: EXO1
• Molecular Weight: Observed: 115kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EXO1, exonuclease 1, HEX1, hExoI, rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1, EXOI

Western blot analysis of extracts of various cell lines, using EXO1 Antibody (orb1258378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human esophageal cancer using EXO1 antibody (orb1258378) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using EXO1 antibody (orb1258378) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using EXO1 antibody (orb1258378) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

EXO1

UniProt

Q9UQ84