EXO1 Antibody

• Catalog Number: orb1258378
• Category: Antibodies
• Description: EXO1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 601-846 of human EXO1 (NP_006018.4).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 115kDa
• Target: EXO1
• UniProt ID: Q9UQ84
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: EXO1, exonuclease 1, HEX1, hExoI, rad2 nuclease fa
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EXO1 Antibody (orb1258378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human esophageal cancer using EXO1 antibody (orb1258378) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using EXO1 antibody (orb1258378) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using EXO1 antibody (orb1258378) at dilution of 1:100. Blue: DAPI for nuclear staining.