ETV6 Antibody

• Catalog Number: orb1259033
• Category: Antibodies
• Description: ETV6 Antibody
• Target: ETV6
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ETV6 (NP_001978.1).
• UniProt ID: P41212
• MW: Observed: 53kDa
• Tested applications: IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ETV6, TEL, TEL/ABL
• Research Area: Cancer Biology, Epigenetics & Chromatin
• Note: For research use only

Western blot analysis of extracts of various cell lines, using ETV6 antibody (orb1259033) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunoprecipitation analysis of 200 ug extracts of SW620 cells using 3 ug ETV6 antibody (orb1259033). Western blot was performed from the immunoprecipitate using ETV6 antibody (orb1259033) at a dilution of 1:500.