ETS1 Antibody (N-term)

• Catalog Number: orb1938761
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB24587
• Tested applications: FC, IF, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 50408 Da
• Target: This ETS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the N-terminal region of human ETS1.
• UniProt ID: P14921
• NCBI: NP_001137292.1, NP_001155894.1, NP_005229.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protein C-ets-1, p54, ETS1, EWSR2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-ETS1 Antibody (N-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: 293T whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of ETS1 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

ETS1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.