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Estrogen Receptor-a(Phospho-Ser167) Antibody

SKU: orb14798

Description

Estrogen Receptor-α (Phospho-Ser167) Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC, WB
Dilution RangeWB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:200
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenPeptide-KLH. Peptide sequence around phosphorylation site of serine 167 (L-A-S(p)-T-N) derived from Human Estrogen Receptor-a
TargetEstrogen Receptor-a
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration1.0mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ER; ESR; ESR1; ESTR; ESTRA

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
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Kie Honjo, Taiji Hamada, Takuya Yoshimura, Seiya Yokoyama, Sohsuke Yamada, Yan-Qin Tan, Lai K. Leung, Norifumi Nakamura, Yasuyo Ohi, Michiyo Higashi, Akihide Tanimoto PCP4/PEP19 upregulates aromatase gene expression via CYP19A1 promoter I.1 in human breast cancer SK-BR-3 cells Oncotarget, 9, 29619 (2018)

Applications

WB

Reactivity

Human

Available Sizes

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50 μl
$ 260.00
100 μl
$ 360.00
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Free Secondary Antibody (20 ul)0/0

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