ESR2 Antibody

SKU: orb1252769

Description

ESR2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep
• Application Notes:
  Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: ERb455
• Immunogen: The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.
• Target: ESR2
• Purification: Protein G purified monoclonal antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2

Quick Database Links

Gene Symbol

ESR2

UniProt

Q92731