Estrogen Receptor beta Antibody [NR3Gb-1]

• Catalog Number: orb1252088
• Category: Antibodies
• Description: Estrogen Receptor beta Antibody [NR3Gb-1]
• Target: ESR2
• Clonality: Monoclonal
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G purified
• Immunogen: The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.
• UniProt ID: Q92731
• Tested applications: FC, IF, IHC, WB
• Application notes: Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: NR3Gb-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Estrogen receptor beta, ER-beta, Nuclear receptor
• Research Area: Cancer Biology, Metabolism Research, Neuroscience,
• Note: For research use only

IHC testing of ovarian cancer stained with Estrogen Receptor beta antibody.

IHC testing of gastric carcinoma stained with Estrogen Receptor beta antibody.

FACS testing of MCF-7 cells: Black = cells alone; Green = isotype control; Red = Estrogen Receptor beta antibody PE conjugate.