ESR1 Antibody

SKU: orb1251942

Description

ESR1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human
• Application Notes:
  Western blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Estrogen Receptor antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: SPM567
• Immunogen: Recombinant human Estrogen Receptor alpha protein (amino acids 2-185) was used as the immunogen for this Estrogen Receptor alpha antibody.
• Target: ESR1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Estrogen Receptor antibody (SPM567).

Western blot of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor antibody (SPM567).

Quick Database Links

Gene Symbol

ESR1

UniProt

P03372