ESR1 Antibody (Biotin)

SKU: orb751052

Description

Mouse monoclonal antibody against ESR1 conjugated to Biotin

Research Area

Cancer Biology, Cardiovascular Research, Epigenetics & Chromatin, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: IHC
• Dilution range: Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: MCF-7 cells. Human breast or endometrial carcinoma

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: ESR1/3373
• Immunogen: Recombinant full-length human Estrogen Receptor alpha protein
• Target: ESR1
• Source/Expression System: Bioreactor
• Molecular Weight: ~67kDa
• Purification: Protein A
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Disclaimer: For research use only

Alternative Names

Anti-Estrogen Receptor alpha delta 4*5 antibody, anti-6 antibody, anti-7*/654 isoform antibody, anti-Estrogen Receptor alpha delta 4 +49 isoform antibody, anti-Nuclear receptor subfamily 3 group A member 1 antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÂ’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÂ’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373).

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373).

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373).

SDS-PAGE Analysis Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

ESR1

UniProt

P03372

NCBI Gene ID

2099