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Catalog Number | orb699547 |
---|---|
Category | Antibodies |
Description | This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ESR1/3565 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | A portion of amino acids 129-312 from the human protein was used as the immunogen for the Estrogen Receptor alpha antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This Estrogen Receptor alpha antibody is available for research use only. |
UniProt ID | P03372 |
Storage | Store the Estrogen Receptor alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
FACS, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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