ESR1 Antibody

SKU: orb1253804

Description

ESR1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Estrogen Receptor-a (Phospho-Ser104) antibody was raised against a peptide sequence around phosphorylation site of serine 104 (S-V-S (p) -P-S) derived from Human Estrogen Receptor-α.
• Target: ESR1
• Molecular Weight: 66 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ER, ESR, Era, ESRA, ESTRR, NR3A1, SHC, SHCA, ER, ESR1, ESTR, ESTRA

Western blot analysis of lysed extracts from MCF-7 cells untreated or treated with E2 and EGF using Estrogen Receptor-α (Phospho-Ser104).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Phospho-Ser104).

Quick Database Links

Gene Symbol

ESR1

UniProt

P03372

RefSeq (Protein):NP_000116.2