Estrogen Inducible Protein pS2 / TFF1 Antibody

• Catalog Number: orb248549
• Category: Antibodies
• Description: This antibody recognizes a polypeptide of ~6.5kDa, identified as Estrogen Inducible Protein pS2. The antibody's epitope is localized between amino acids 57-84. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. Estrogen Inducible Protein pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for estrogen inducible protein pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that estrogen inducible protein pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GE2
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen Inducible Protein pS2 was used as the immunogen for this antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Estrogen Inducible Protein pS2 antibody is available for research use only.
• Entrez: 7031
• Storage: Store the Estrogen Inducible Protein pS2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Inducible Protein pS2 antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1mg/ml in PBS for 15 min at 37oC. Heat Induced Epitope Retrieval is not suitable for this antibody.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast cancer tissue using Estrogen Inducible Protein pS2 antibody