CES3 Antibody (HRP)

SKU: orb344899

Description

Esterase antibody (Peroxidase)

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, WB: 1:250 - 1:1,000
• Reactivity: Porcine
• Application Notes:
  Anti-Esterase Peroxidase conjugated antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Esterase [Porcine Liver]
• Target: CES3
• Purity: Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver]. Cross reactivity against Esterase from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Esterase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase

Western Blot of Rabbit anti-Esterase Antibody Peroxidase Conjugated. Lane 1: Esterase (Porcine Liver). Load: 50 ng per lane. Primary antibody: Rabbit anti-Esterase Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: n/a. Block: orb348637 for 30 min at RT. Predicted/Observed size: 62 kDa, 55 kDa for Esterase.

Quick Database Links

Gene Symbol

CES3

UniProt

Q29550

RefSeq:343791015