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Catalog Number | orb1294314 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Estrogen Receptor alpha |
Target | ESR1 / Estrogen receptor alpha |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit-IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Immunogen | Synthetic peptide / Extracellular domain corresponding to Human Estrogen Receptor alpha |
Tested applications | IHC, WB |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:200 – 1:250 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Estrogen Receptor alpha antibody at 1/800 dilution, Lysates at 60 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294314 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.