ESR1 Rabbit Polyclonal Antibody

SKU: orb1294314

Description

Rabbit polyclonal antibody to Estrogen Receptor alpha

Research Area

Cancer Biology

Images & Validation

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Item 1 of 5

Tested Applications	IHC, WB
Dilution Range	Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:200, Immunohistochemistry (Paraffin) 1:200 – 1:250
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / Extracellular domain corresponding to Human Estrogen Receptor alpha
Target	ESR1 / Estrogen receptor alpha
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Estrogen Receptor alpha antibody at 1/800 dilution, Lysates at 60 µg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294314 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.

Quick Database Links

Gene Symbol

ESR1 / Estrogen receptor alpha

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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