ESR1 Antibody

SKU: orb241921

Description

This ESR1 Antibody is an unconjugated polyclonal product. It tagets ESR1 using a Recombinant Human Estrogen receptor protein (10-591AA) as the immunogen. This antibody is suitable for ChIP, ELISA, IHC, WB. Purification: >95%, Protein G purified.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ChIP, ELISA, IHC, WB
• Dilution Range: WB:1:1000-1:5000, IHC-P:1:20-1:200
• Reactivity: Human, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant Human Estrogen receptor protein (10-591AA)
• Target: ESR1
• Purification: >95%, Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ER-alpha antibody, anti Estradiol receptor antibody, anti Nuclear receptor subfamily 3 group A member 1 antibody

Chromatin Immunoprecipitation MCF-7 (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-ESR1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers again

Western blot aanalysis of Hela whole cell lysate (Lane1), MCF-7 whole cell lysate (Lane2), Rat brain tissue (Lane3), Colo320 whole cell lysate (Lane4)using Estrogen R antibody

Immunohistochemistry of paraffin-embedded human breast cancer tissue using Estrogen R antibody

Quick Database Links

Gene Symbol

ESR1

UniProt

P03372