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Item 1 of 4
Item 1 of 4
ESPN Antibody (N-term)
Catalog Number: orb1929743
| Catalog Number | orb1929743 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB20951 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 91733 Da |
| Target | This ESPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human ESPN. |
| UniProt ID | B1AK53 |
| NCBI | NP_113663.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Espin, Autosomal recessive deafness type 36 protei Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ESPN Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human testis tissue reacted with ESPN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ESPN Antibody (N-term) in 293 cell line lysates (35 ug/lane). ESPN (arrow) was detected using the purified Pab.
Anti-ESPN Antibody (N-term) at 1:1000 dilution + SK-BR-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 92 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
