Recombinant Erythropoietin Antibody / EPO

SKU: orb2643528

Description

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. [UniProt]

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting points. Titration of the Erythropoietin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: ERTP-1
• Immunogen: Amino acids 28-162 were used as the immunogen for the recombinant Erythropoietin antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human kidney with recombinant Erythropoietin antibody (clone ERTP-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

P01588