ERV3 Antibody

• Catalog Number: orb1249005
• Category: Antibodies
• Description: ERV3 Antibody
• Target: ERV3
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-PQDNFTLTASS
• UniProt ID: Q14264
• Tested applications: ELISA, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.9kDa according to NP_001007254.2. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml. This product has been successfully used in IHC on Human: Frese S et al, PLoS One. 2015 Jul 8;10(7). PMID: 26154387.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERV3, endogenous retroviral sequence 3 (includes z
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_001007254.2

orb1249005 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.