ERp57 Antibody

• Catalog Number: orb396316
• Category: Antibodies
• Description: Mouse Anti-Human ERp57 Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4F9
• Tested applications: ICC, IF, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ICC/IF (1:100)
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: ERp57
• Entrez: 2923
• UniProt ID: P30101
• NCBI: NP_005304.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: ERp60 Antibody, ERp61 Antibody, Grp57 Antibody, Gr
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-266 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ERp57 Antibody. (D) Composite.

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 57 kDa.