ERP57 Antibody

SKU: orb11608

Description

Goat polyclonal to ERp57 - endoplasmic reticulum lumen marker. This endoplasmic reticulum protein interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. It has disulfide isomerase activity and complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000
• Reactivity: Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinante peptide derived from within residues 300 aa to the C-terminus of human ERP57 produced in E. coli. Antigen Sequence: STAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRESDFISYLQREATNPPVIQEEKPKKKKKAQEDL
• Target: PDIA3 protein disulfide isomerase family A, member 3
• Purification: Epitope affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Concentration: Please inquire
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

58 kDa glucose regulated protein, 58 kDa microsomal protein, disulfide isomerase ER 60, endoplasmic reticulum resident protein 57, endoplasmic reticulum resident protein 60, ER protein 57, ER protein 60, ER protein 61, ERP57, ERp60, ERp61, glucose regulated protein 58 Kd, GRP57, GRP58, HsT17083, P58, PDIA3, phospholipase C alpha, PI PLC, protein disulfide isomerase A3 antibody.

Western blot analysis of TR-iBRB and Jurkat cell lysates using ERP57 antibody

Confocal immunofluorescence analysis of RPE cells using ERP57 antibody

Quick Database Links

Gene Symbol

PDIA3 protein disulfide isomerase family A, member 3