ERp57 Antibody (HRP)

SKU: orb396329

Description

Mouse monoclonal antibody against ERp57 conjugated to HRP.

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB (1:1000); ICC/IF (1:100)
• Reactivity: Human
• Application Notes:
  A 1:1000 dilution of SMC-266 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4F9
• Immunogen: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)
• Target: ERp57
• Molecular Weight: 57 kDa
• Purification: Protein G Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ERp60, ERp61, Grp57, Grp58, P58, PDIA3, PI PLC, 58 kDa glucose regulated protein, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER 60, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, ER p57, ER protein 57, ER protein 60, ERp 57, ERp57, Glucose Regulated Protein 58 Kd, GRP 57, GRP 58, GRP57, HsT17083, p58, PDIA 3, PDIA3_HUMAN, Phospholipase C alpha, Protein disulfide isomerase A3, Protein disulfide isomerase family A member 3, Protein disulfide-isomerase A3

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ERp57 Antibody. (D) Composite.

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 57 kDa.

Quick Database Links

Gene Symbol

ERp57

UniProt

P30101

NCBI Gene ID

2923

RefSeq (Protein):NP_005304.3