ERK5 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559213
• Category: Antibodies
• Description: ERK5 Recombinant Rabbit Monoclonal Antibody
• Target: MAPK7
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human ERK5 protein, around C-terminal 200aa
• UniProt ID: Q13164
• MW: 90 kDa
• Tested applications: ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-100, IF=1:100-500, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
• Antibody Type: Recombinant Antibody
• Clone Number: 8D8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1
• Note: For research use only

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-ERK5 antibody (orb559213), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF-7, 2: 293T, 3: NIH/3T3, 4: PC-, 12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 88 kDa, Observed MW: 115 kDa.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERK5) Polyclonal Antibody, Unconjugated (orb559213) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Testis (Mouse) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-ERK5 (orb559213) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 110 kD.