Erk2 Monoclonal Antibody [Knockout Validated]

• Catalog Number: orb1787822
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: WB
• Predicted Reactivity: Other, Rat
• Reactivity: Human, Mouse
• Isotype: IgG1,k
• Immunogen: 154-183 aa
• Dilution range: WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 41390 Da
• Target: This Erk2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from human Erk2.
• UniProt ID: P28482
• NCBI: NP_002736.3, NP_620407.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mitogen-activated protein kinase 1, MAP kinase 1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Erk2 Antibody (Center) western blot analysis in MCF-7, HepG2, NCI-H292, Hela, mouse NIH/3T3 and Neuro-2a, rat C6 cell line lysates (35μg/lane). This demonstrates the MAPK1 antibody detected the MAPK1 protein (arrow).

All lanes: Anti-Erk2 Antibody at 1:1000 dilution (upper). Lane 1: Hela. Lane 2: Hela-Erk2-Knockout. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.