ERK2 Internal Antibody HRP

SKU: orb420477

Description

ERK1/2 antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:40,000, WB: 1µg/mL
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-ERK 2 (RABBIT) antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-ERK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
• Purity: This HRP conjugated affinity purified antibody is directed against human ERK2 protein. Anti-ERK2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK1 isoform.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ERK2 antibody Peroxidase conjugation, HRP conjugated rabbit anti-ERK2 antibody, MAPK1, ERK 2, ERK-2, P42MAPK, PRKM1, PRKM2, MAPK 2, MAP kinase 2, Mitogen-activated protein kinase 2, p42-MAPK, MAP kinase isoform p42, Extracellular signal-regulated kinase 2, ERT1, MAP kinase 1, Mitogen-activated protein kinase 1

Quick Database Links

UniProt

P28482