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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p44/42 MAPK. |
| Target | MAPK3/MAPK1 |
| Molecular Weight | Calculated MW: 42,44 kDa; Observed MW: 42,44 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of ERK1/2 in COLO205 lysates using ERK1/2 antibody.

Western blot analysis of ERK1/2 in K562 lysates using ERK 1/2 antibody.

Western blot analysis of ERK1/2 in HELA lysates using ERK 1/2 antibody.
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Protocol Information
ERK1/2 Rabbit pAb (orb1562987)
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