ErbB2 (HER2) Rabbit mAb [KD Validated] Antibody

SKU: orb1727627

Description

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB:1:500~1:2000 IHC:1:50~1:200 ICC/IF:1:50~1:200 IP:1:50 FC:1:50
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human ErbB2 (HER2)
• Molecular Weight: 185kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C-erbB-2; ErbB2; HER2, MLN 19; NEU; NEU proto-oncogene; NGL; Receptor protein-tyrosine kinase erbB-2;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of SKBR cells, using ErbB2 (HER2) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ErbB2 (HER2) Antibody.

Western blot analysis of ErbB2 (HER2) expression in SKBR-3 cell lysate.

Quick Database Links

UniProt

P04626