EPRS Antibody (N-term)

SKU: orb38139

Description

Purified rabbit polyclonal antibody.

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB14633
• Immunogen: Synthetic Peptide
• Target: EPRS1
• Molecular Weight: 170591
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Bifunctional aminoacyl-tRNA synthetase antibody, anti-Proliferation-inducing gene 32 protein antibody, anti-Glutamyl-tRNA synthetase antibody, anti-Prolyl-tRNA synthetase antibody, anti-EPRS antibody, anti-GLNS antibody, anti-PARS antibody, anti-QARS antibody, anti-QPRS antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-EPRS Antibody (N-term) at 1:8000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 171 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

EPRS1

UniProt

P07814

RefSeq (Protein):NP_004437.2

Mendes, Marisa I. et al. Bi-allelic Mutations in EPRS, Encoding the Glutamyl-Prolyl-Aminoacyl-tRNA Synthetase, Cause a Hypomyelinating Leukodystrophy Am J Hum Genet, 102, 676-684 (2018)

PubMed: 29576217