EPO Antibody (Biotin)

• Catalog Number: orb1671424
• Category: Antibodies
• Description: EPO Antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: EPO
• UniProt ID: P01588
• NCBI: P01588
• Storage: Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Erythropoietin, Epoetin
• Note: For research use only
• Application notes: Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human EPO as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human EPO. Western Blot To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Human EPO by sandwich ELISA (using 100 μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human EPO as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human EPO.

To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.