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Catalog Number | orb1930013 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19629 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 68222 Da |
Target | This EPN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-153 amino acids from the N-terminal region of human EPN3. |
UniProt ID | Q9H201 |
NCBI | NP_060427.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Epsin-3, EPS-15-interacting protein 3, EPN3 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of MDA-231 cells using EPN3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human colon carcinoma with EPN3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
EPN3 Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the EPN3 antibody detected the EPN3 protein (arrow).