Epitope Tag Protein Marker Lysate

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Catalog Number: orb348609

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Product Overview

Product Name	Epitope Tag Protein Marker Lysate
Catalog Number	orb348609
Hazard Information	Non-Toxic
Target	Epitope Tag Protein Marker Lysate
Alternative Names	(click to expand) Flag, MBP, HA, 6XHIS, Lysate

Product Properties

Form/Appearance	Liquid (in 1x Loading Buffer)
Storage	Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note	For research use only.
Purity	Loading control for Western blotting. Lysate expresses 12 different epitope tags

Product Description

Epitope Tag Protein Marker Lysate

Application Notes

Application Notes	This product is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E.coli lysate was prepared by lysing cells in loading buffer, heating to 95° C for 10' followed by centrifugation at 13,000 g for 15'. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 μl is recommended for western blotting. The product is supplied in 1X loading buffer and is ready-to-use. 62.5 mM Tris HCl, 10% Glycerol, 2% SDS, 0.05% bromphenol blue, pH 6.8 + 1% Triton 100 + 2.5 mM EDTA+5mM Tris HCl+4% (v/v) beta -Mercaptoethanol.

This product is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E.coli lysate was prepared by lysing cells in loading buffer, heating to 95° C for 10' followed by centrifugation at 13,000 g for 15'. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 μl is recommended for western blotting. The product is supplied in 1X loading buffer and is ready-to-use. 62.5 mM Tris HCl, 10% Glycerol, 2% SDS, 0.05% bromphenol blue, pH 6.8 + 1% Triton 100 + 2.5 mM EDTA+5mM Tris HCl+4% (v/v) beta -Mercaptoethanol.

Validation Images

Western blot analysis of 12-Epitope Tag Protein Marker lysate using Epitope Tag Protein Marker Lysate

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