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Epitope Tag Protein Marker Lysate

Epitope Tag Protein Marker Lysate

Catalog Number: orb348609

DispatchUsually dispatched within 5-10 working days
$ 320.00
Catalog Numberorb348609
CategoryProteins
DescriptionEpitope Tag Protein Marker Lysate
Tested applicationsWB
Concentration1.0 mg/ml
Dilution rangeWB: User Optimized
Form/AppearanceLiquid
PurityLoading control lysate made in E. Coli for Western blotting expresses 12 different epitope tags.
ConjugationUnconjugated
Hazard InformationNon-Toxic
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.
Buffer/PreservativesNone. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Alternative namesFlag-tag marker, MBP-tag marker, HA-tag marker, 6X
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NoteFor research use only
Application notesThis 12 Epitope Tag Protein Marker Lysate has been tested in western blot and is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for western blotting.
Expiration Date6 months from date of receipt.
  • 12 Epitope GST Tag Protein Marker Lysate [orb535115]

    SDS-PAGE,  WB

    Unconjugated

    Loading control lysate made in E. Coli for Western blotting expresses 12 different epitope tags.

    100 μl
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