12 Epitope Tag Control Lysate (GST)

SKU: orb535115

Description

12 Epitope GST Tag Protein Marker Lysate

Key Properties

• Source/Expression System: E. coli
• Purity: Control lysate made in E. coli for Western blotting expresses 12 different epitope tags.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid
• Buffer/Preservatives: Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Flag-tag marker, GST-tag marker, HA-tag marker, 6XHIS-tag marker, T7-tag marker, cMyc-tag marker, V5-tag marker, S-tag marker, HSV-tag marker, VSV-tag marker, Glu-Glu-tag marker, E-tag marker, Lysate marker, Protein Marker, Loading control, epitope tag marker, positive control marker

SDS-Page of 12 Epitope GST Tag Protein Lysate. Lane 1: Opal Molecular Weight. Lane 2: 0.5 µg lysate. Lane 3: 1.0 µg lysate. Lane 4: 2.0 µg lysate. Lane 5: 3.0 µg lysate. Lane 6: 5.0 µg lysate.

Western Blot of GST tag- 12 epitope protein marker lysate. Lane 1: 7 µl load of molecular weight marker. Lane 2: 10 µg load of 12 epitope GST tag protein marker. Antibody: Incubate for 1hr at RT. Lane 2a: Mouse anti-Flag DyLight488 at 1:40000. Lane 2b: Mouse anti-6xHis DyLight488 at 1:40000. Lane 2c: Mouse anti-GST DyLight649 at 1:5000. Lane 2d: Rabbit anti-cMyc DyLight488 at 1:10000. Lane 2e: Rabbit anti-HA DyLight649 at 1:10000.

Western Blot of GST tag- 12 epitope protein marker lysate. Lane 1: 7 µl load of molecular weight marker. Lane 2: 5 µg load of 12 epitope GST tag protein marker. Antibody: Incubate for 1hr at RT. Lane 2a: Mouse anti-Flag DyLight488 at 1:40000. Lane 2b: Mouse anti-6xHis DyLight488 at 1:40000. Lane 2c: Mouse anti-GST DyLight649 at 1:5000. Lane 2d: Rabbit anti-cMyc DyLight488 at 1:10000. Lane 2e: Rabbit anti-HA DyLight649 at 1:10000.