EphB4 Antibody

• Catalog Number: orb1929076
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14731
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 108270 Da
• Target: This EphB4 antibody is generated from rabbits immunized with human recombinant EphB4 protein.
• UniProt ID: P54760
• NCBI: NP_004435.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ephrin type-B receptor 4, Hepatoma transmembrane k
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EphB4 (arrow) using rabbit polyclonal EphB4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB4 gene (Lane 2).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the EphB4 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.