EphA3 Antibody (C-term)

• Catalog Number: orb1929101
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB1585
• Tested applications: IHC-P, WB
• Reactivity: Hamster, Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 110131 Da
• Target: This EphA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-928 amino acids from the C-terminal region of human EphA3.
• UniProt ID: P29320
• NCBI: NP_872585.1, NP_005224.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ephrin type-A receptor 3, EPH-like kinase 4, EK4,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-EphA3 Pab in CHO cell lysate. EphA3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of EphA3 (arrow) using rabbit polyclonal EphA3 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA3 gene (Lane 2).

Formalin-fixed and paraffin-embedded human placenta tissue reacted with EphA3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.