EphA1 rabbit pAb

• Catalog Number: orb765145
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EphA1
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human EPHA1. AA range:541-590
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 108
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-EPHA1 antibody, anti-EPH antibody, anti-EPHT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using EphA1 Polyclonal Antibody.

Immunofluorescence analysis of HeLa cells, using EPHA1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 cells, using EPHA1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).