Ep-CAM Antibody

SKU: orb750082

Description

EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P
• Dilution Range: ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Canine, Feline, Human
• Application Notes:
  Titering of the Ep-CAM antibody may be required for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: EGP40/1798
• Immunogen: A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of human MCF-7 cells with Ep-CAM antibody (green, clone EGP40/1798) and Reddot nuclear stain (red).

Western blot testing of human MCF7 cell lysate with Ep-CAM antibody (clone EGP40/1798). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Ep-CAM antibody (clone EGP40/1798). These results demonstrate the foremost specificity of the EGP40/1798 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Ep-CAM antibody (clone EGP40/1798) as confirmation of integrity and purity.

IHC testing of FFPE human colorectal carcinoma and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

IHC testing of FFPE dog bladder and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

IHC testing of FFPE cat small intestine and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

Quick Database Links

UniProt

P16422